Figure 5

Results of P815 cell culture and intervention experiments.
Western blotting analysis of KCa3.1 levels (KCa3.1/β-actin) and phosphorylation of AKT (p-AKT/AKT) (a–c) tryptase mRNA levels assayed by real-time PCR (d). Concentrations of IL-6 and IL-18 in the culture supernatants (e–f). One-way ANOVA test was employed for intergroup comparison. *P < 0.01 vs blank control group. #P < 0.01 vs Ksh group. *P > 0.05 vs blank control group. The cropped blots images are shown in the figure and the full-length blots images are presented in Supplementary Information.