Figure 2
The effect of Zn2+ limitation on P. aeruginosa growth.
The growth phenotypes of P. aeruginosa PAO1 (blue) and the ΔznuA strain (green) were compared by absorbance at 600 nm. (a) CDM overnight seed cultures were supplemented with 10 μM TPEN, prior to growth analyses in unsupplemented CDM. (b) CDM overnight seed cultures were supplemented with 10 μM TPEN, prior to growth analyses in CDM supplemented with 10 μM TPEN. (c) CDM overnight seed cultures were supplemented with 10 μM TPEN, prior to growth analyses in CDM supplemented with 30 μM TPEN. (d) CDM overnight seed cultures were supplemented with 10 μM TPEN, prior to growth analyses in CDM supplemented with 60 μM TPEN. Cultures were grown as indicated and, in all experiments, data are the mean ± s.e.m., with n ≥ 3.
