Figure 2

Structure of TARS-BC194 complex.

(a) Two-dimensional scheme of TARS-BC194 interactions. H-bonding residues are shown as sticks. Hydrophobic interacting residues are shown in grey. (b) Structure superimposition of TARS-BC194 (green) and TARS-BN (grey) complexes. The protein is shown in ribbon cartoon representation and the bound BC194 and BN are shown as pink and blue sticks, respectively. (c) Close up view of BC194 (green) and BN (grey) binding site residues. The five shared H-bonds are shown as black dash lines. The 2 BN-specific interactions are shown as blue dash lines, while the corresponding distances in BC194 structure are indicated in pink. (d) Close up view of threonine binding interactions. Interactions are shown as dashed lines. (e,f) The effects of BC194 (e) and BN (f) treatment on a cell-free translation system. Rabbit reticulocyte lysate (RRL) was incubated with 0.02 mg/ml luciferase mRNA and translation of luciferase enzyme was quantified in a luminescence assay. Serial diluted BC194 and borrelidin (2.5 nM - 25 μM) was added to inhibit the translation of luciferase mRNA; mean ± SEM, n = 3.