Figure 1

LIF and LIFRα expression in wild-type mouse implantation sites and placenta throughout gestation.

Wild type (WT) mouse implantation sites were collected from n = 3 mice/timepoint. (a) LIF, (b) LIFRα and (c) gp130 mRNA expression were analysed in E10, 13, 15 and 17 placenta by quantitative real-time PCR, normalized to β2-microglobulin. Data are mean ± SEM, ANOVA, Tukey’s post-hoc test, *p < 0.05, **p < 0.01, ***p < 0.001. (d) LIF protein expression was analysed in E6, 8, 10 implantation sites and E13, 15 and 17 placenta by Western blot, normalized to GAPDH. (e) Data are mean ± SEM, ANOVA, Tukey’s post-hoc test, *p < 0.05, **p < 0.01, ***p < 0.001.