Figure 4

LIF inhibition during placental development results in abnormal placental morphology in mice.

E10, 13 or 17 implantation sites treated with PEG control or PEGLA from E8–10, E10–13 or E10–17 were stained with haematoxylin-eosin (H&E). (a) At E10 (b) E13 and (c) E17, low and high power images of the implantation sites are shown. Arrows denote trophoblast giant cells (top panel, bars represent 500 μm, lower panel, bars represent 50 μm). (b) At E13, the deciua (D), spongiotrophoblast (yellow outline, S) and labyrinth (L) are denoted (top panel, bars represent 500 μm, lower panel, bars represent 500 μm). (d–f) At E13 and E17, the total placental, spongiotrophoblast (junctional zone, JZ) and labyrinth area (mm²) were calculated using CellSense software. At least 3 mid-sagital sections of implantation sites per mouse (n = 4/group) were analysed. Data are mean ± SEM, students t-test, *p < 0.05.