Figure 6

LIF blockade during placentation increases trophoblast area at mid-, but not late-gestation.

Representative photomicrographs of (a) E13 or (b) E17 implantation sites treated with PEG control or PEGLA from E10–13 or E10–17 stained for cytokeratin. (c) Cytokeratin staining intensity was analysed using CellSense software and quantified as staining intensity (pixels) per area (%) in at least 4 fields of 3 mid-sagital implantation site sections per mouse (n = 4/group) and averaged. Bars represent 50 μm, inset is negative control. Data are mean ± SEM, students t-test, **p < 0.01.