Figure 7

LIF inhibition during placental development impairs placental vasculature development and alters mouse placental angiogenic/vascular and oxidative stress gene transcription.

Representative photomicrographs E13 or 17 implantation sites treated with PEG control or PEGLA from E10–13 or E10–17 stained for (a) Isolectin-B4 to highlight extracellular matrix surrounding maternal sinusoid branches, which were counted (b), or (c) CD31 immunostaining to highlight fetal vessel endothelial cells. Bar represents 100 μm. Insets are negative controls. (d) The fetal vessel diameter was measured in at least 4 fields of 3 mid-sagital implantation site sections per mouse (n = 4/group) and averaged. Data are mean ± SEM, students t-test, ***p < 0.001. (e–f) Candidate genes from a PCR array screen (Supp Table 1) on E13 PEG or PEGLA mouse placenta were validated by semi-quantitative PCR and normalized to β2-microglobulin (n = 4/group). Data are mean ± SEM, students t-test, *p < 0.05, ***p < 0.001.