Figure 1
From: Self protein-protein interactions are involved in TPPP/p25 mediated microtubule bundling
TPPP/p25 induces microtubule polymerization.
(a) Schematic representation of the various TPPP/p25 fragments used in this study. The ΔN(49) fragments has the first 49 residues deleted whereas the ΔC(158) fragment has residues 158–219 deleted; core fragment lacks both residues 1–49 and 158–219. (b) Coomasie-stained gel of bacterially expressed and purified His-tagged TPPP/p25 fragments [FL = full length TPPP/p25; ΔN = ΔN(49); ΔC = ΔC(158)]. (c) Light scattering assays (OD350 nm) of tubulin solutions (15 μM) in the presence of equimolar concentrations of each of the four TPPP/p25 constructs. (d) EM images of microtubules assembled from tubulin solutions (15 μM) in the absence or presence of 15 μM or 30 μM of each of the four different TPPP/p25 fragments (scale bars = 50 nm).
