Figure 1

Kinexus Western phospho-microarray analysis and Western blotting of mCRP-induced signalling in BAEC.

A shows quantitative Kinexus phospho-protein screening array carried out on BAEC after exposure to mCRP (8 minutes) demonstrated up-regulation of several potentially important proteins that may be implicated in AD pathology including Tau (2.3 fold) Focal adhesion kinase and IRS-1 (3.4 fold). IRS-1 was investigated in more detail in our in vitro studies Fig. 1B shows by Western blotting in the same samples, that mCRP induced approximately a fourfold increase in p-IRS expression compared with control untreated cells (bar chart). P-Tau was also increased by approximately 5-fold (C) and in addition, we showed that the cellular content of Aβ1–42 increased 3-fold over 24 h whilst PEN-2 also increased (2-fold; 8 minutes). These experiments were carried out at least twice and a representative example is shown.