Figure 3 | Scientific Reports

Figure 3

From: The Multiple DSF-family QS Signals are Synthesized from Carbohydrate and Branched-chain Amino Acids via the FAS Elongation Cycle

Figure 3

The effects of leucine and valine on DSF biosynthesis.

(a) Effect of total amino acids and total non-branched-chain amino acids on DSF-family signals production. XYSA: XYS medium supplemented with 100 μM total amino acids; XYSN: XSY medium supplemented with 100 μM non-branched-chain amino acids. (b) HPLC analysis of DSF and BDSF production by the ΔrpfC strain in media XYS (as indicated in black line), XYSL (red line) and XYSV (blue line) at 24 h after inoculation. XYSL: XYS medium supplemented with 50 μM leucine. XYSV: XYS medium supplemented with 350 μM valine. (c) The dose–response curve between leucine or valine concentration and DSF production. (d) DSF production time course of the ΔrpfC strain in media XYS, XYSL and XYSV. Data are means ± one standard deviation of three independent assays. Different letters indicate significant differences between treatments (LSD at P = 0.05).

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