Figure 4

The effects of isoleucine on IDSF biosynthesis.
(a) HPLC analysis of DSF, BDSF and IDSF production in media XYS and XYSI. XYSI: XYS medium supplemented with 50 μM isoleucine. (b) The dose–response curve between isoleucine concentrations (10 to 75 μM) and IDSF production. (c) IDSF production time course of the ΔrpfC strain in XYSI medium. Data are means ± one standard deviation of three independent assays.