Figure 2
From: MicroRNA-107 contributes to post-stroke angiogenesis by targeting Dicer-1
miR-107 enhances tubular formation and migration of RBMECs and HUVECs in vitro.
(A) Top: Representative photomicrographs of tube formation of HUVECs transfected with miR-107 or scr-miR and un-transfected HUVECs (control). Tube formation was measured after 12 hours under normoxia. Bottom: Tube formation capacity of HUVECs transfected with anti-miR-107 or scr-miR and un-transfected HUVECs (sham). The anti-miR-107 effect was investigated under OGD for 12 h. Total magnification, ×100 (B) the number of tubule branches per field. (C) The cumulative sprout length per field. (D–F) RBMECs (G) Top: RBMECs transfected with miR-107 or scr-miR and un-transfected RBMECs (control) were incubated on a Transwell system under normoxia. Bottom: RBMECs transfected with anti-miR-107 or scr-miR and un-transfected RBMECs (sham) were incubated on a Transwell system under OGD. The number of migrating cells was determined after 12 hours. (H) Quantification of the migration is expressed as the number of migrating cells per high-power field. (I,J) HUVECs. (K) The effect of miR-107 overexpression under normoxia on HUVECs migration was determined by scratch wound assay. Wound closure was determined after 12 hours. White lines indicate edges of scratch wounds. Representative photomicrographs showed migration. (L) Quantization of HUVECs migration. Data are presented as mean ± SD. *P < 0.05, vs. control group under normoxia, #P < 0.05, vs. sham group subjected to OGD.
