Figure 7
Overexpression of VP1 also promotes GM130 degradation and disrupts Golgi ribbons in HeLa cells.
(a) HeLa cells were transfected with VP1 plasmids or infected with or without CVB3 for varied times as indicated. GM130 and VP1 were detected by immunofluorecent staining and visualized by confocal microscopy. Bar = 10 μm. (b) HeLa cells were transfected with empty vector or VP plasmid for 24 hours. The expression of GM130 in the cell lysates was examined by Western blot with GM130 antibody. Actin was also probed to serve as a loading control. The relative GM130 protein level was analyzed and shown on the right of the blots. *P < 0.01 vs vector group.
