Figure 2
From: Left brain cortical activity modulates stress effects on social behavior
Recovery of sociability in susceptible mice by photostimulation of left mPFC neurons.
(a) Timeline of optogenetic stimulation experiments. (b) Infection pattern of AAV2/9-CamKII-hChR2(H143R)-mCherry in the mPFC. A schematic of mPFC is drawn guided by Franklin and Paxinos mouse brain atlas. (c) Multiunit recordings following stimulation with 473-nm laser pulses of 5, 10 and 20 Hz. (d) Upper: Representative heat map indicating the location of susceptible mice without (OFF) or with (ON) stimulation in the presence of a social target. Left panel: Behavior of susceptible mice stimulated in the left mPFC (Susceptible-L). Right panel: Behavior of susceptible mice stimulated in the right mPFC (Susceptible-R). Lower: Comparison of social-interaction time in the presence of a social target among susceptible mice receiving left or right stimulation of the mPFC (Left, n = 5; Right, n = 5). Left stimulation restored social interaction in susceptible mice, whereas right stimulation had no significant effect. Left OFF vs. Left ON, t4 = −12.556, *P = 0.0002, paired t-test; Right OFF vs. Right ON, t4 = −2.533, P = 0.064, paired t-test; Left ON vs. Right ON, t8 = 3.129, *P = 0.014, t-test. Data are presented as means ± s.e.m. (e) Upper: Representative heat map indicating the location of resilient mice without (OFF) or with (ON) stimulation in the presence of a social target. Left panel: Behavior of resilient mice stimulated in the left mPFC (Resilient-L). Right panel: Behavior of resilient mice stimulated in the right mPFC (Resilient-R). Lower: Comparison of social-interaction time in the presence of a social target among resilient mice receiving left or right stimulation of the mPFC (Left, n = 6; Right, n = 5). Left stimulation decreased social interaction in the resilient phenotype, whereas right stimulation did not significantly change social interaction. Left OFF vs. Left ON, t5 = 4.384, *P = 0.007, paired t-test; Right OFF vs. Right ON, P = 0.625, Wilcoxon signed rank test; Left ON vs. Right ON, t9 = −2.479, *P = 0.035, t-test. Data are presented as means ± s.e.m.
