Table 2 Summary of CRISPR/Cas9-induced site-specific insertion with ssODN through HDR.

From: Efficient CRISPR/Cas9-mediated biallelic gene disruption and site-specific knockin after rapid selection of highly active sgRNAs in pigs

Injected components

No. of tested blastocysts

HDR blastocysts (% of tested)

Precise HDR (% of tested)

Cas9 mRNA + R1 sgRNA + ssODN (80 ng/μL)

23

3 (13.04)

2 (8.70)

Cas9 mRNA + R1 sgRNA + ssODN (10 ng/μL)

20

0

0

Cas9 mRNA + F2 sgRNA + ssODN (80 ng/μL)

19

0

0

  1. Different pools as indicated were coinjected into mature MII pig oocytes. After parthenogenetic activation, oocytes were cultured to the blastocyst stage and a single blastocyst was selected for genotyping by RFLP and Sanger sequencing assays.
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