Table 3 Summary of CRISPR/Cas-mediated gene targeting in porcine primary fibroblasts.

From: Efficient CRISPR/Cas9-mediated biallelic gene disruption and site-specific knockin after rapid selection of highly active sgRNAs in pigs

sgRNA

No. of tested colonies

Monoallelic mutants (% of tested)

Biallelic mutants (% of tested)

R1

17

1 (5.88)

7 (41.18)

R2

10

2 (20.00)

0 (0)