Figure 2 | Scientific Reports

Figure 2

From: Integrated Transcriptomics Establish Macrophage Polarization Signatures and have Potential Applications for Clinical Health and Disease

Figure 2

In vitro validation of selected genes from M(IFNγ + LPS, TNFα) and M(IL-4, IL-13) signatures.

(A) RT-qPCR from human MDM activated with 50 ng/mL of M-CSF for 7 days and stimulated with IFNγ (20 ng/mL) + LPS (100 ng/mL) or IL-4 (20 ng/mL) for additional 18 h. (B) RT-qPCR from THP-1 (human acute monocyte leukemia cell line) differentiated with 20 ng/mL PMA for 3 days and stimulated with IFNγ (20 ng/mL) + LPS (100 ng/mL) or IL-4 (20 ng/mL) for additional 24 h. Data represent median and IQR (interquartile range) of five independent experiments normalized to TATA binding box protein (TBP). Data was considered statistically significant for *(P ≤ 0.05) and ** (P ≤ 0.01) (Mann-Whitney U test).

Back to article page