Figure 2 | Scientific Reports

Figure 2

From: Chimeric peptide constructs comprising linear B-cell epitopes: application to the serodiagnosis of infectious diseases

Figure 2

Biomarkers for serodiagnosis.

Both antigen and antibodies can be the target of detection. (a) P. falciparum specific antigen can be detected by sandwiched immunoassay to indicate infection. (b) Epitopes are immunodominant regions of antigen protein, which can interact with antibodies. Antigens were divided into overlapped peptides (30 amino acids in length with 15 amino acids overlapped), which were printed on iPDMS membranes to form microarrays. (c) A heat map of 304 serums (179 P. falciparum infected, 125 healthy) to 2038 peptides was obtained by directly converting SNR to grayscale. (d) In large-scale seroscreening, specific antibodies-epitope interactions resulted in high signal intensity region on the heat map. (e) Representative result from P. falciparum infected serum. (f) Representative result from healthy serum.

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