Figure 1
From: Dynamic subnuclear relocalisation of WRKY40 in response to Abscisic acid in Arabidopsis thaliana
Subnuclear localisation of WRKY18, WRKY40 and WRKY60.
Confocal images of A. thaliana protoplast (Col-0) transformed with either YFP-WRKY18 (A) or YFP-WRKY40 (B) or YFP-WRKY60 (C) (scale bar 10 μm). (D) DAPI staining of nuclei expressing WRKY18-YFP and YFP-WRKY40 in transiently transformed A. thaliana seedlings (scale bar 10 μm). Bar charts show changes in subnuclear localisation in response to ABA for WRKY18 from nuclear bodies to nucleoplasm in Col-0 (E) and for WRKY40 in Col-0 (F), ost1–4 mutant (G) and snrk2.2/2.3/ost1–4 mutant (H) protoplasts (means ± SE). Statistical analysis was performed with Fisher Exact test (P < 0.05). Asterisks indicate significant difference when significance was obtained in at least 3 biological replicates.
