Figure 3

Wdr13 is important for junctional and labyrinth zone development.
(A) Gross histology of 17.5 dpc placenta (H&E staining) shows reduced placental size in Wdr13 mutant placenta as compared to wild type. (B) Periodic acid-Schiff (PAS) staining of 17.5 dpc placenta shows reduced junctional zone in Wdr13 mutant placenta as compared to wild type. (C) Magnified image of PAS staining. (D) Masson’s trichrome staining of 17.5 dpc placenta shows increased maternal blood space and reduced number of trophoblast giant cells in labyrinth zone of Wdr13 mutant placenta as compared to wild type. Filled arrowhead shows fetal endothelial cells and closed arrowhead show S-TGC. (E) Quantification of number of sinusoidal trophoblast giant cells in labyrinth zone of Wdr13 mutant and wild type placenta (n = 4). (F) Quantification of maternal blood space (MBS) in labyrinth zone of Wdr13 mutant and wild type placenta (n = 4). (G) Expression of various genes involved in glucose and amino acid transport, angiogenesis and differentiation (AP 1 target genes) in 17.5 dpc Wdr13 mutant and wild type placenta (n = 4–6).