Figure 10

Sox2-expressing glial cells in adult auditory nerves have the properties of neural stem/progenitor cells.

(a) Schematic structure of the Sox2-eGFP transgene. (b,c) Glial cells expressed GFP signifying Sox2 expression (arrows). PI⁺ neuron nuclei ((b), red) or NF200⁺ neuronal cell bodies ((c), red) were surrounded by GFP⁺ glial cells (green). (d) A large proportion of neurosphere cells expressed GFP (green). (e–g) Neurospheres propagated under neural differentiation regimen express neuronal and glial cell markers. Scale bars, 10 μm in (b,c); 45 μm in (e); 45 μm in (f,g).