Figure 4

Gene expression profiles in injured auditory nerve recapitulate aspects of auditory nerve postnatal development.

(a) Expression profiles of genes differentially expressed in adult auditory nerves at 3 days and 7 days after ouabain injury was shown in the left panel. 6915 genes were identified by the criteria of fold changes >1.5 and p < 0.05 (Student’s unpaired t-test) at either 3 days or 7 days after ouabain exposure. Expression profiles of genes differentially expressed in postnatal auditory nerves are present in the right panel. 6023 genes were identified as significantly different by the criteria of fold changes >1.5 and p < 0.05 (Student’s paired t-test) at either P3, P7 or P10 compared to P0. Colorimetric scaling (Z-standardization) is indicated at the bottom. (b) A Venn diagram showed that many of the genes differentially expressed following ouabain exposure are also differentially expressed during postnatal development (2061 of 6915). (c) A heat map displayed expression profiles of 636 genes that were up-regulated in adult auditory nerves by injury and also highly expressed at the onset of auditory nerve development (P0) but down-regulated during postnatal development. (d) Functional categorization of the 636 genes depicted in panel (c).