Figure 1 | Scientific Reports

Figure 1

From: Sandcastle: software for revealing latent information in multiple experimental ChIP-chip datasets via a novel normalisation procedure

Figure 1

Representation of the ChIP-chip procedure.

Proteins are crosslinked to chromatin (a) which is extracted, sonicated and split into two samples. IP is carried out on one sample to separate out the chromatin bound to the factor of interest (b). Both samples are purified to DNA, amplified by PCR and differentially labelled (c). They are allowed to hybridise to the microarray probes and the resulting intensity values from the scanned image (d) are converted to numerical values which can be plotted (e) and processed as required by the investigation. Figure created and drawn by Mark Bennett.

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