Figure 1 | Scientific Reports

Figure 1

From: Real-time analysis of epithelial-mesenchymal transition using fluorescent single-domain antibodies

Figure 1

Selection of single-domain antibodies (nano-/chromobodies) against vimentin.

(a) Amino acid sequence alignment of eight unique vimentin-specific VHH/VH domains (nanobodies, Nbs) identified after two rounds of biopanning followed by phage ELISA. (b) Representative images of HeLa cells expressing selected sdAbs fused to GFP (vimentin chromobodies). Cells stained with an anti-vimentin antibody (α-VIM-IgG) or expressing GFP served as controls. Scale bar: 20 μm.

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