Table 1 Nanobodies bind vimentin with high affinities.

From: Real-time analysis of epithelial-mesenchymal transition using fluorescent single-domain antibodies

Nbs

KD [nM]

kon[1/Ms]

koff [1/s]

R2

VB3

11.25

6.0 ± 0.11 × 105

6.7 ± 0.16 × 10−3

0.98

VB6

37

4.75 ± 0.14 × 105

1.76 ± 0.06 × 10−2

0.98

  1. The table summarizes affinities (KD), association (kon) and dissociation constants (koff) determined for VB3 and VB6. Corresponding sensograms are shown in Supplementary Figure 4.
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