Figure 1
From: Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative
Effect of sonorensin on S. aureus biofilms.
(a) Inhibition of attachment of S. aureus cells by sonorensin at various concentrations (b) Inhibition of biofilm formation (c) Viability of S. aureus cells biofilm (assayed by XTT). Control bars indicate S. aureus cells without any treatment taken as 100% in case of a & c and 0% in case of b. Each well of 96 -well plate contains 4 × 106 CFU S. aureus cells and variable concentration of sonorensin in 200 μl of BHI-sucrose. The plates were incubated at 37 °C for 4 h and 24 h for biofilm attachment assay (a) and inhibition of biofilm formation (b), respectively. (a) About 1.8 ± 0.05% attachment of biofilm was observed in the presence of 1X MIC of sonorensin. (b) Sonorensin showed significant inhibitory activity against S. aureus biofilm formation at 24 h, (c) Reduced XTT conversion was observed in wells with higher sonorensin concentrations and the control (without treatment) showed maximum reduction of XTT. The results were presented as mean ± SD and differences between the control and treated samples were statistically significant (n = 3) (p < 0.005).
