Figure 4

JMJD3 cooperates with RUNX2 to promote osteoblast differentiation in vivo.

(A) Representative skeletal preparation, von Kossa staining and in situ hybridization withCol1a1 and Bglap probes of Jmjd3^+/+; Runx2^+/+, Jmjd3^+/−; Runx2^+/+, Jmjd3^+/+; Runx2^+/−and Jmjd3^+/−; Runx2^+/− mice. Red arrows: most delayed bone ossification in parietal bone and clavicles of Jmjd3^+/−; Runx2^+/− mice at E15.5. Black arrow: most delayed mineralization in perichondrium of humerus of Jmjd3^+/−; Runx2^+/− mice at E15.5. Blue arrow: most reduced Col1a1 mRNA level in perichondrium of humerus of Jmjd3^+/−; Runx2^+/− mice at E14.5. Purple arrow: most reduced Bglap mRNA level in perichondrium of humerus of Jmjd3^+/−; Runx2^+/− mice at E18.5. Littermates at each group were compared. n = 3. Scale bar: 1 mm in a–h; 200 μm in i–t. (B) Quantification of width of parietal bones (a), Col1a1 (b) and Bglap (c) mRNA level of humerus perichondrial osteoblasts of Jmjd3^+/+; Runx2^+/+, Jmjd3^+/−; Runx2^+/+, Jmjd3^+/+; Runx2^+/− and Jmjd3^+/−; Runx2^+/− embryos at E15.5 (a), E14.5 (b) or E18.5 (c) respectively. *p < 0.05. Error bar represents the SE of three independent experiments.