Figure 5
S1PR2/3 antagonists attenuate the hepatic inflammation in cholestatic liver injury.
(a) Serum ALT and AST activity were measured using automatic biochemistry analyzer. (b) The mRNA level of F4/80 in liver was measured by real-time RT-PCR. (c) The mRNA levels of inflammatory cytokines in liver. Inflammatory cytokines proteins in liver tissues of mice with different treatments were analyzed by BD Cytometric Bead Array. (d) Representative scatter plots with six bead populations, which represent IL-6, IL-10, MCP-1, IFN-γ, TNFα and IL-12p70, respectively, based on APC fluorescence intensity from high to low. (e) The quantitative results of CBA essay. *P < 0.05 vs. Control. #P < 0.05 vs. BDL group (n = 7 per group).
