Figure 3
Typical optical recording of a single AP.
The AP was distributed in the phloem region. (a) The ΔF/F curves are plotted on the left side. Each curve represents the change in the fluorescence intensity of the region around it with a spatial size of about 20 μm × 20 μm. From the signal curves numbered I, II, III and IV, similar ΔF/F waveform and amplitude can be observed and in the whole region, the maximum amplitude of the ΔF/F signal is about 8%. On the right side, the time lapse ∆F/F images, are presented. The stimulus was applied at the fifth second. Following the stimulus, the ∆F/F change can be clearly observed in the phloem region. (b,c) The ∆F/F curves and the time lapse ∆F/F images of the other two samples. All of the images indicate that the AP is mainly distributed in the phloem region.
