Figure 4
Infarct size and neurological function in NOX2 and WT mice.
Mice of both genders and between 6–20 weeks old were subjected to tMCAO. (A) TTC stainings of three sequential coronal brain sections on day 1 after tMCAO that were representative for each subgroup: young (6–10 week old), adult (18–20 week old) male and adult female NOX2 KO and WT mice. The TTC staining colors viable tissue red, while infarcted tissue stays white. (B) Bar graphs of mean infarct volumes ± SEM from WT (open bar) and NOX2 KO mice (closed bars). (C) Scatter plot and median of neurodeficit Bederson scores on day 1 after tMCAO, ranging from 0 (normal) to 5 (severe), from WT (open symbols) and NOX2 KO mice (closed symbols). (D) Scatter plot and median of grip test scores, ranging from 0 (severe deficit) to 5 (normal) from WT (open symbols) and NOX2 KO mice (closed symbols). The asterisk, *, indicates statistical significance (P < 0.05, t-test) for the infarct size in the subgroup adult male NOX2 versus WT; however, this group was insufficiently powered to allow the detection of a difference. Combined analysis did not show a significant difference for any of the parameters.
