Table 1 Power analysis of experimental stroke studies validation the role of NOX2 in Nox2-/y and WT mice.

From: A combined pre-clinical meta-analysis and randomized confirmatory trial approach to improve data validity for therapeutic target validation

Reference

Gender

Duration ischemia (min)

Time of sacrifice (h)

Infarct size

Power for 40% effect

NOX2 WT

NOX2 KO

N

Mean (mm 3)

SD

CV

N

Mean (mm 3)

SD

CV

Reperfusion time 24 h, short ischemia

 Brait38

M

30

24

8

58,63

32,16

0,55

10

30,04

28,37

0,94

26

 

F

30

24

7

24,93

17,04

0,68

10

42,69

17,01

0,40

24

 De Silva35

M

30

24

15

36,30

13,20

0,36

7

14,70

10,20

0,69

28

Reperfusion time 24 h, middle long ischemia

 Chen40

M

75

24

5*

53,90

19,23

0,36

5*

26,00

10,73

0,41

54

 Chen41

M

75

24

3*

50,00

7,79

0,16

3*

28,50

3,93

0,14

29

Kleinschnitz24

M

60

24

10

78,70

19,50

0,25

18

79,18

20,90

0,26

95

 McCann53

M

60

6

5

12,90

4,30

0,33

5

9,50

2,10

0,22

59

 

M

60

24

5

35,60

4,30

0,12

6

15,10

3,10

0,21

54

Reperfusion time 24 h, long ischemia

 Liu49

M

90

24

5

38,00†

2,00

0,05

5

25,00†

5,00

0,20

16

 Kahles37

M

120

24

13

83,88

41,92

0,50

14

43,20

38,23

0,88

41

 Tang36

M

120

24

6

163,83

35,59

0,22

4

69,41

35,66

0,51

15

 Walder23

M

120

24

10

54,00

33,52

0,62

13

29,10

20,19

0,69

35

 Wang42

M

120

24

8

35,25

9,50

0,27

8

19,38

8,55

0,44

25

Reperfusion time 72 h

 Chen40

M

75

72

5*

106,20

27,95

0,26

5*

52,00

26,39

0,51

25

 Chen41

M

75

72

3*

86,00

16,00

0,19

3*

55,00

16,16

0,29

14

 Kunz39

M

25

72

5*

52,00

24,40

0,47

5*

20,10

11,70

0,58

25

 McCann53

M

90

72

5

28,77

14,14

0,49

8

20,92

9,28

0,44

31

Reperfusion time 24 h, middle long ischemia, including current study

 Chen40

M

75

24

5*

53,90

19,23

0,36

5*

26,00

10,73

0,41

48

 Chen41

M

75

24

3*

50,00

7,79

0,16

3*

28,50

3,93

0,14

26

Kleinschnitz24

M

60

24

10

78,70

19,50

0,25

18

79,18

20,90

0,26

92

 McCann53

M

90

6

5

12,90

4,30

0,33

5

9,5

2,10

0,22

53

 

M

90

24

5

35,60

4,30

0,12

6

15,10

3,10

0,21

48

 Multicentre

F

60

24

10

99,77

33,12

0,33

19

75,44

32,99

0,44

97

 

M

60

24

21

76,76

10,53

0,14

14

84,66

9,31

0,11

93

 

All

60

24

41

89,00

32

0,36

51

80,00

31,00

0,39

100

  1. To assess the power of already published studies, original data sets were requested from the authors. Means, SD’s and numbers of animals were extracted from the original data or, if original data sets could not be obtained, from the text or figures using ‘universal desk top ruler’ software. If only the SEM was reported, the SD was calculated using formula (2).
  2. (2).
  3. For four different groups, pooled variances were used to calculate the power for detecting a 40% difference was calculated using the Russ Lenth’s power and sample size software (http://homepage.stat.uiowa.edu/~rlenth/Power/index.html). The power for the ‘reperfusion time 24 h, middle long ischemia’ group were recalculated using the newly available data from the current pRCT study. * Lowest number of number range † Infarct size reported as percentages.
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