Table 3 Enzymatic kinetic parameters of wild-type and mutated CatAs after purification against the substrate catechol*.

From: Engineering catechol 1, 2-dioxygenase by design for improving the performance of the cis, cis-muconic acid synthetic pathway in Escherichia coli

Enzymes

Km(μM)

kcat(S−1)

WT

7.88 ± 1.239

7.86 ± 0.248

L73F

151.09 ± 10.299

5.65 ± 0.163

P76A

65.91 ± 7.753

46.56 ± 0.201

L73F P76A

45.96 ± 7.691

39.50 ± 0.221

  1. *WT indicates wild-type CatA. Others are mutated proteins. Errors (standard deviations) were calculated based on at least three independent experiments.
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