Figure 1

Prostate anatomy, sample preparation and ATR-FTIR or Raman spectroscopy.
(A) Prostate anatomy illustrating the different histological zones. TURP removes part of the transition zone. (B) Low-E slide containing a prepared sample. (C) Micrograph of a prostate sample as visualised during Raman spectroscopy. (D) H&E stained section for histological comparison and to ensure no diathermy artefacts contaminate the sample. (E) Unprocessed ATR-FTIR spectral dataset (x-axis: wavenumbers (cm−1), y-axis: absorbance) (F) Unprocessed Raman spectral dataset. (x-axis: wavenumbers (cm−1), y-axis: intensity).