Figure 3 | Scientific Reports

Figure 3

From: Enhancing flavonoid production by systematically tuning the central metabolic pathways based on a CRISPR interference system in Escherichia coli

Figure 3

A CRISPRi-based strategy for targeting gene identification.

All target genes were silenced with high efficacy. It was found that sgRNAs targeting ppsA, eno, glyA, adhE, mdh, fumC, sdhABCD, sucC, cite, fabH, fabB, fabF and fabI showed dramatic increases in acetyl-CoA concentration (increased by over 180%). Meanwhile, sgRNAs targeting ppsA, eno, adhE, mdh, fumC, sdhA, sucC, cite, fabH, fabB, fabF and fabI showed dramatic increases in acetyl-CoA and malonyl-CoA concentrations (increased by over 223%). 1 mL of cell culture was harvested at the mid-log phase of growth to quantify the intracellular concentrations of malonyl-CoA and acetyl-CoA.

Back to article page