Figure 4
Tuning target gene expression to balance cell proliferation and malonyl-CoA accumulation.
To achieve high, medium or low silencing efficacy toward each target gene, sgRNA bound the non-template DNA strand of the target gene at the initial, intermediate or terminal gene coding region. (A) Target genes were repressed with high silencing efficacy. (B) Target genes were repressed with medium silencing efficacy. (C) Target genes were repressed with low silencing efficacy. 1 mL of cell culture was harvested at the mid-log phase of growth to quantify the intracellular concentration of malonyl-CoA. Final OD600 values of cultures were measured after a total fermentation time of 48 h.
