Figure 5 | Scientific Reports

Figure 5

From: Enhancing flavonoid production by systematically tuning the central metabolic pathways based on a CRISPR interference system in Escherichia coli

Figure 5

Effects of single or multiple genetic perturbations on (2S)-naringenin production.

Control strains contained the (2S)-naringenin heterologous pathway without an RNA-guided dCas9:sgRNA system. The sgRNA-expressing plasmids repressing single or multiple genes were further transformed into the control strain to investigate the effects of these systems on (2S)-naringenin production. Final OD600 values, average specific growth rates and concentrations of p-coumaric acid and (2S)-naringenin were measured from production strains after a total fermentation time of 48 h.

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