Table 1 Primers and vectors used for single gene perturbations.

From: Enhancing flavonoid production by systematically tuning the central metabolic pathways based on a CRISPR interference system in Escherichia coli

  1. aEach primer pair shares the same reverse primer Pr_sgRNA (at the end of the Table).
  2. b‘High’, ‘Medium’ and ‘Low’ mean constructed vectors with high, medium and low silencing efficacy toward target genes, respectively.
Back to article page