Table 2 Primers used for multiple gene perturbations.

From: Enhancing flavonoid production by systematically tuning the central metabolic pathways based on a CRISPR interference system in Escherichia coli

Oligonucleotides

Sequences (5′-3′) *

Pf_sgRNA(BamHI)

CGCGGATCCTGTACACTGCAGGTCGTAAATCAC

Pr_sgRNA(EcoRI)

CCGGAATTCAAAAAAGCACCGACTCGGTG

Pf_sgRNA(EcoRI)

CCGGAATTCTGTACACTGCAGGTCGTAAATCAC

Pr_sgRNA(HindIII)

CCCAAGCTTAAAAAAGCACCGACTCGGTG

Pf_sgRNA(HindIII)

CCCAAGCTTTGTACACTGCAGGTCGTAAATCAC

Pr_sgRNA(NdeI)

GGAATTCCATATGAAAAAAGCACCGACTCGGTG

Pf_sgRNA(NdeI)

GGAATTCCATATGTGTACACTGCAGGTCGTAAATCAC

Pr_sgRNA(BglII)

GAAGATCTAAAAAAGCACCGACTCGGTG

Pf_sgRNA(BglII)

GAAGATCTTGTACACTGCAGGTCGTAAATCAC

Pr_sgRNA(KpnI)

GGGGTACCAAAAAAGCACCGACTCGGTG

  1. *Bold and underlined letters are restriction enzyme cut sites.
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