Table 3 Plasmids used for multiple gene perturbations.

From: Enhancing flavonoid production by systematically tuning the central metabolic pathways based on a CRISPR interference system in Escherichia coli

Plasmids

Description

Sources

pCDFDuet-1

Double T7 promoters, CDF ori, SmR

Novagen

pETDuet-1

Double T7 promoters, pBR322 ori, AmpR

Novagen

pACYCDuet-1

Double T7 promoters, P15A ori, CmR

Novagen

pCOLADuet-1

Double T7 promoters, ColA ori, KnR

Novagen

pCDF-Trc-TAL-Trc-4CL

pCDFDuet-1 carrying TAL and 4CL under Trc promoter

24

pET-CHS-CHI

pETDuet-1 carrying CHS and CHI

24

pACYC-dCas9

pACYCDuet-1 carrying dCas9

This study

pCOLA-fabF(high)/adhE(low)

Vectors with high silencing efficacy toward fabF and low silecncing efficacy toward adhE

This study

pCOLA-fabF(high)/eno(low)

Vectors with high silencing efficacy toward fabF and low silecncing efficacy toward eno

This study

pCOLA-fabF(high)/mdh(low)

Vectors with high silencing efficacy toward fabF and low silecncing efficacy toward mdh

This study

pCOLA-fabF(high)/fabB(low)

Vectors with high silencing efficacy toward fabF and low silecncing efficacy toward fabB

This study

pCOLA-fabF(high)/fumC(medium)

Vectors with high silencing efficacy toward fabF and medium silecncing efficacy toward fumC

This study

pCOLA-fabF(high)/sucC(medium)

Vectors with high silencing efficacy toward fabF and medium silecncing efficacy toward sucC

This study

pCOLA-fabF(high)/fumC(medium)/adhE(low)

Vectors with high, medium and low silencing efficacy toward fabF, fumC and adhE, respectively

This study

pCOLA-fabF(high)/fumC(medium)/eno(low)

Vectors with high, medium and low silencing efficacy toward fabF, fumC and eno, respectively

This study

pCOLA-fabF(high)/fumC(medium)/fabB(low)

Vectors with high, medium and low silencing efficacy toward fabF, fumC and fabB, respectively

This study

pCOLA-fabF(high)/fumC(medium)/sucC(medium)

Vectors with high, medium and medium silencing efficacy toward fabF, fumC and sucC, respectively

This study

pCOLA-fabF(high)/fumC(medium)/fabB(low)/adhE(low)

Vectors with high, medium, low and low silencing efficacy toward fabF, fumC, fabB and adhE, respectively

This study

pCOLA-fabF(high)/fumC(medium)/fabB(low)/eno(low)

Vectors with high, medium, low and low silencing efficacy toward fabF, fumC, fabB and eno, respectively

This study

pCOLA-fabF(high)/fumC(medium)/fabB(low)/sucC(medium)

Vectors with high, medium, low and medium silencing efficacy toward fabF, fumC, fabB and sucC, respectively

This study

pCOLA-fabF(high)/fumC(medium)/fabB(low)/sucC(medium)/adhE(low)

Vectors with high, medium, low, medium and low silencing efficacy toward fabF, fumC, fabB, sucC and adhE, respectively

This study

pCOLA-fabF(high)/fumC(medium)/fabB(low)/sucC(medium)/eno(low)

Vectors with high, medium, low, medium and low silencing efficacy toward fabF, fumC, fabB, sucC and eno, respectively

This study

pCOLA-fabF(high)/fumC(medium)/fabB(low)/sucC(medium)/adhE(low)/eno(low)

Vectors with high, medium, low, medium, low, low and low silencing efficacy toward fabF, fumC, fabB, sucC, adhE and eno, respectively

This study

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