Figure 1
From: Super-resolution mapping of glutamate receptors in C. elegans by confocal correlated PALM
Labeling strategy for confocal correlated PALM.
(a) Schematic overview of the C. elegans nervous system, showing the major neuronal ganglia and processes. Ganglia containing GLR-1 expressing neurons are marked in magenta. L1 larvae are approximately 250 μm long and 25 μm in diameter, but grow to 1.3 mm long and 80 μm diameter when reaching the adult stage. (b) Genomic position of the glr-1-gene on chromosome III. Boxes represent the exons, while the connecting bars represent the introns. Two amplicons were obtained by genomic PCR: the first containing the glr-1 gene with approximately 4 kb of putative promoter sequence upstream from the start codon and the second containing only the putative promoter region. The former was fused to the sequence encoding mEOS2 while the latter was placed in front of the eGFP-encoding sequence. (c) Confocal image showing a partial Z-projection of the right lateral side of transgenic C. elegans expressing both the Pglr-1::glr-1::mEOS2 and the Pglr-1::eGFP constructs. The GLR-1 expressing neurons are represented in cyan, while the localization of the GLR-1-mEOS2 fusion protein is coded in magenta. Annotated neurons indicated by white arrows. Scale bar measures 2 μm.
