Figure 4
From: Super-resolution mapping of glutamate receptors in C. elegans by confocal correlated PALM
Confocal correlated PALM in C. elegans.
(a) Partial confocal Z-projection (15 optical slices, total thickness of 5.30 μm) of eGFP fluorescence marking GLR-1 positive neurons and their processes (blue) overlaid with transmission image of the C. elegans head region (grey). White arrowheads indicate the nerve ring and the VNC. (b) Enlargement of GLR-1 expressing head neurons from Z-projection in (a). (c,d) Close up of the AVEL neuron (c) and the nerve ring (d) indicated in (b) with the distribution of GLR-1 mapped onto the neuron by ccPALM. (e,f) Close up of box in panels (c) and (d), respectively. Scale bars indicate 2 μm (a,b) 250 nm (c,d), or 100 nm (e,f). Cut-off resolution of PALM images (c–f) is 20 nm.
