Figure 2
Water-soluble metabolic profiling in fecal samples of the human gut-derived microbiota (HGM) mice.
(a) Principal component analysis (PCA) of fecal 1D 1H-NMR spectroscopy profiles. The contributions of PC1, PC2 and PC3 were 63.7%, 20.1% and 6.3%, respectively. (b) Fecal pimelate, butyrate and acetate levels at day 13. All compounds were calculated and identified using 1H-13C HSQC 2D-NMR measurements (n = 6). Boxes denote the interquartile range between the first and third quartiles and the line within denotes the median. P-values were calculated using Mann–Whitney U test. *P < 0.05; **P < 0.01. (c) Biotin concentrations in fecal samples at day 13. Data are shown as the mean ± SD (n = 6). P-values were calculated using Student’s t-test. *P < 0.05. (d) Correlation analysis based on Spearman’s rank correlation coefficient between fecal pimelate and biotin levels. Fecal pimelate and biotin levels are shown as open symbols and the line indicates a simple regression model.
