Figure 4 | Scientific Reports

Figure 4

From: Binding interface between the Salmonella σS/RpoS subunit of RNA polymerase and Crl: hints from bacterial species lacking crl

Figure 4

BACTH interaction analyses between Crl from S. Typhimurium and σS wild-type and variant proteins from S. Typhimurium and P. aeruginosa.

(a) Interaction between CrlSTM-T18 and the T25-σS hybrid proteins indicated was quantified by measuring β-galactosidase activity in Miller units. Results are the mean of at least three independent experiments and standard deviations are indicated with black bars. (b) Immunodetection of T25-σS fusion proteins by antibodies directed against the T25 polypeptide. Lane 1, σSSTM; lane 2, σSPA; lane 3, σSPA H83Y; lane 4, σSPA L87R; lane 5, σSPA Q89L; lane 6, σSPA K90R; lane 7, σSSTM; lane 8, σSSTM R82L; lane 9, σSSTM R82E.

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