Figure 3 | Scientific Reports

Figure 3

From: EGFR-L858R mutant enhances lung adenocarcinoma cell invasive ability and promotes malignant pleural effusion formation through activation of the CXCL12-CXCR4 pathway

Figure 3

The EGFR-L858R mutant increases expression of the chemokine receptor, CXCR4.

(a) CXCR4 mRNA expression in H1299-EGFR-WT and H1299-EGFR-L858R cells was quantified by real-time RT-PCR (*P < 0.01). (b) CXCR4 mRNA expression in CL1-0-EGFR-WT and CL1-0-EGFR-L858R cells was quantified by real-time RT-PCR (*P < 0.01). (c) CXCR4 mRNA expression in H1299-EGFR-L858R cells after transfection with EGFR-L858R–specific siRNA or scrambled siRNA control was quantified by real-time RT-PCR (*P < 0.01). (d) H1299-EGFR-WT and H1299-EGFR-L858R cells were serum-starved for 24 hours and then treated with different concentrations of EGF (10, 40 and 100 ng/ml) for 2 hours. After EGF stimulation, CXCR4 surface expression was evaluated by flow cytometry. Data are presented as means ± SD of three independent experiments. P-values (two-sided) were determined using Student’s t-test.

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