Table 3 Parameters and critical time points for safety assessment.
From: Effects of acoustic levitation on the development of zebrafish, Danio rerio, embryos
Parameter | Low humidity (LH) group | Medium-low humidity (MLH) group |
---|---|---|
Humidity (RH %) | 13.3 | 28.9 |
Room temperature (°C) | 22.8 | 21.9 |
Number of levitated samples (unless specified) | 6 | 6 |
Initial sample volume (E3 droplet + embryo) (μL) | 18.0 ± 4.3 | 24.2 ± 3.3 |
Initial embryo volume (μL) | 0.9 ± 0.1 | 0.9 ± 0.0 |
Mortality at 12–14 hpf | 100 | 100 (n = 5) |
Time (t1) when sample height (E3 droplet + embryo) = embryo diameter (s) | 2056 ± 667 | 3359 ± 793 |
Time (t2) when sample volume (E3 droplet + embryo) = embryo volume (s) | 2990 ± 375 | 4985 ± 482 |
Time (t3) when embryo spontaneously exited the levitator (s) | 3428 ± 18 (n = 2) | 4735 (n = 1) |
Time (t4) when embryo dried and was manually extracted from levitator (s) | 3569 ± 19 (n = 4) | 5578 ± 391 (n = 5) |
Samples failures at insertion | 1 | 0 |
Samples failures at extraction | 0 | 1 |