Figure 6
From: High expression of SOX30 is associated with favorable survival in human lung adenocarcinoma
Analyses of cell proliferation, cell cycle and apoptosis associated with SOX30 over-expression in NCI-H520 cells.
(A) Transfectants of SOX30 and vector control in NCI-H520 cells were identified by WB. Abundant SOX30 was detected after SOX30 transfection but not after the vector control transfection. Error bars indicate s.d. (n = 3). (B) MTS assays were used to examine the effect of SOX30 on cell proliferation in NCI-H520 cells. Cell viability was evaluated in triplicate by CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega). (C) Cell-cycle profiles were determined by flow cytometry. (D) Cell apoptosis was performed by flow cytometry assay with Annexin V-APC/7-amino-actinomycin D double staining in NCI-H520 cells. Three independent experiments were performed.
