Figure 2

T cell activation in the presence of IL-11-induced CD11b⁺CD14⁺ cells.

CD11b⁺CD14⁺ cells were generated from PBMCs in the presence or absence of IL-11 for 7 days. Isolated CD11b⁺CD14⁺ cells were co-cultured with CFSE-labelled, donor-matched autologous CD4⁺ and CD8⁺ T cells with or without T-cell receptor (TCR) stimulation by CD3/CD28 beads. (a) At 3 days after stimulation, T cell proliferation was determined by flow cytometric analysis of CFSE dilution. Representative histograms of the fluorescence intensity for CD4⁺ and CD8⁺ T cells without stimulation (control) or TCR-stimulated T cells cultured with or without CD11b⁺CD14⁺ cells. (b) IFN-γ production by T cells at 72 h after CD3/CD28 stimulation was measured by ELISA. The means and SDs of the data from three independent experiments are shown. *p < 0.05, compared with control, two-sided Student’s t test.