Figure 2

Schematic representation of artificial gene clusters used for vanillin production.

A T7 promoter and ribosomal binding site precede each gene and a T7 terminator is located downstream of each gene cluster. Module one contains the biosynthetic gene cluster used for ferulic acid production. Module two contains the gene cluster used for production of vanillin from ferulic acid. Module three was used for l-tyrosine overproduction. Cm^r, chloromycetin resistance; Amp^r, ampicillin resistance; Kan^r, kanamycin resistance.