Figure 3
Fermentative production of ferulic acid and vanillin.
The arrow indicates the time of addition of IPTG (0.2 mM). Data are representative of at least three independent experiments and the error bars indicate the standard deviation. (a) For production of ferulic acid, a recombinant strain harboring sam8-sam5-comt/pACYC (red columns) or tal-hpaBC-comt/pACYC (dark cyan columns) was grown in Luria-Bertani (LB) medium with an additional 2 g/L l-tyrosine. Cell growth (lines; colors the same as those for ferulic acid production) is presented as the optical density at 600 nm. (b) Time course of vanillin production from ferulic acid in E. coli VT-3 cultures. (c) Production of vanillin from l-tyrosine using recombinant strains VT-4 and VT-5. (d) Fermentative production of vanillin from glucose. The control strain is E. coli strain K12 harboring sam8-sam5-comt/pACYC and fcs-ech/pET.
