Figure 5
Relative transcription levels of vanillin biosynthetic pathway genes.
Total RNA of recombinant strains VT-4 was extracted from glucose-based LB medium 8 h after induction with IPTG and transcription levels were determined by quantitative RT-PCR. All values are relative to the expression level of the sam8 gene, which was set at 1. Dark cyan and purple columns indicate the expression levels of the genes involved in the artificial pathways from tyrosine to ferulic acid and from ferulic acid to vanillin, respectively. Results are presented as the average of three repetitions from independent total RNA samples.
